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Thermo Scientific™ Buffer Set for Restriction Enzymes
Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.
Thermo Scientific™ TheraPure™ Restriction Enzymes
Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.
Cut Site | CTCTTC(1/4)^ |
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Content And Storage | Store at -20°C. |
Form | Liquid |
Enzyme | Eam1104I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Quantity | 150 μL |
Type IIS RE | Yes |
Incubator Temperature | 37°C |
Concentration | 20 U/μL |
For Use With (Application) | In vitro Transcription |
Inactivation Temperature | 65°C |
Sensitive to Heat Inactivation | Yes |
Product Type | Restriction Enzyme |
Research Category | Drug Development |
Compatible Buffer | 10X Tango Buffer |
Optimal Reaction Temperature | 37°C |
Product Line | TheraPure™ |
Thermo Scientific™ FastDigest Eco31I (IIs class)
The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Eco31I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ Dilution Buffer for Restriction Enzymes
Optimize applications that require diluted enzymes with this dilution buffer.
Invitrogen™ Anza™ 9 NdeI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Shipping Condition | Approved for shipment on Wet or Dry Ice |
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Sensitive to Heat Inactivation | Yes |
Product Type | Restriction Enzyme Cloning System |
Enzyme | Nde I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | Anza™ 10X Red Buffer, Anza™ 10X Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Product Line | Anza™ |
Thermo Scientific™ Eco31I (BsaI) (10 U/μL)
The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Eco31I (BsaI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer G |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ AarI (2 U/μL)
Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | AarI |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | Unique Buffer (10X Buffer AarI) |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 2 U/μL |
Thermo Scientific™ FastDigest XbaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Xba I |
Methylation Sensitivity | Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest XhoI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Xho I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest KpnI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest HindIII
The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Hind III |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ Eco91I (BstEII) (10 U/μL)
The Eco91I (BstEII) restriction enzyme recognizes G^GTNACC sites and cuts best at 37°C in O buffer (Isoschizomers: BstEII, BstPI, EcoO65I, PspEI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Eco91I (BstEII) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer O |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ FastDigest BshTI
Cut at A^CCGGT sites with Thermo Scientific™ FastDigest™ AgeI (BshTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest SacI
The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | SacI |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 3' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |